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Text File | 1991-09-25 | 4.7 KB | 90 lines | [APPL/LETR] |
- DNA Translator Package consists of the following:
-
- DNA Translator [stack 711K]
- Aligner [stack 84K]
- DNA Translator Intro [help text 18K]
- Sample Input/Output [folder]
- 12S rRNA [folder]
- Samples to input [folder]
- EuGene Input [Sample Data #1 7K]
- Phylip.result Input [Sample Data #2 14K]
- Sample output files [folder]
- EuGene String Output [Sample Data #3 4K]
- Paup Output [Sample Data #4 7K]
- Hennig86 Output [Sample Data #5 4K]
- Phylip Output [Sample Data #6 4K]
- Sturgeon CytB [folder]
- GenBank Format Input [Sample Data #7 4K]
- Nucleotide String Output [Sample Data #8 4K]
- Peptide String Output [Sample Data #9 4K]
- Reading Frame Output [Sample Data #10 7K]
- About Sample Data [help text 20K]
- Version History [help text 39K]
- Sequences [folder -- do not change name or directory]
- BTAMTCG [cow mtDNA 21K]
- DYAMTCG [Drosophila mtDNA 21K]
- GGAMTCG [chicken mtDNA 18K]
- HSAMTCG [human mtDNA 21K]
- MMUMTCG [mouse mtDNA 21K]
- PAUMTCG [Paramecium mtDNA 42K]
- PLIMTCG [Urchin - Paracentrotus mtDNA 21K]
- RNOMTCG [Rat mtDNA 21K]
- SPOMTCG [yeast mtDNA 25K]
- SPUMTCG [Urchin - Strongylocentrotus mtDNA 21K]
- XLAMTCG [frog mtDNA 21K]
- MPOCPCG [liverwort cpDNA - Provided upon request 119K]
- NTACPCG [tobacco cpDNA - Provided upon request 158K]
- OSACPCG [rice cpDNA - Provided upon request 133K]
- Sequence Speaker [stack - Provided upon request 161K]
- Codon Usage [Excel format matrix 109K]
-
- Everything except files listed as "Provided upon request" are distributed in
- compressed form as a self-extracting archive (Compact Pro)
-
-
- About Sample Input/Output Data
-
- To facilitate a first-time user's experience with DNA Translator, I have
- provided two sets of sample data. The first, a portion of small (12S)
- rRNA-encoding gene for four vertebrates and one urchin, contains sample aligned
- data from EuGene ("EuGene Input") [SD #1], which can be directly converted to
- Nexus (PAUP) [SD #3] or string formats [SD #4] by selecting "Aligned Nucleotides..."
- from the Convert Menu and by clicking on the corresponding option in the resulting
- dialog box. The string output file [SD #3] is also suitable as an input file for
- any of the conversions in the dialog box resulting from selecting "String
- Nucleotides..." from the Covert menu, or "Import Multiple" from the File menu of
- the Aligner stack. The PAUP output file [SD #4] likewise can be used as sample input
- to test conversion of Nexus format to either "Hennig86 Output" [SD #5] or
- "Phylip Output" [SD #6] by selecting "Nexus Nucleotides..." from the Convert menu.
- These data files should be ready-to-use for analysis by those programs. If you
- analyze the "Phylip Output" file [SD #6] with PHYLIP 3.3, Phylip will output a
- file which should resemble "Phylip.result Input" [SD #2] which could then be
- reconverted to string [SD #3], PAUP [SD #4] or Hennig86 [SD #5] formats.
-
- The second folder contains a single GenBank™ sequence ("GenBank Format Input")
- [SD #7] saved by screen capture as it appeared on the screen while logged onto the
- Wisconsin GCG package via remote terminal. Select "Nucleotide Strings..." from the
- Convert Menu and select the radio button for converting a GenBank formatted file
- to a string sequence ("Nucleotide String Output") [SD #8], which can then be
- translated into formatted reading frame(s) as in "Reading Frame Output" [SD #10]
- or as "Peptide String Output" [SD #9]. You are not limited to single sequences,
- and if you have accumulated several peptide strings that are close to being aligned,
- you can save them "As Is" by selecting "String Peptides" from the Convert menu,
- and "Import Multiple" from the File menu of the Aligner stack. You could also
- interleave the strings from "String Peptides" in the Convert menu, with or without
- dashes for indicating match characters (base pairs that match the first
- interleaved sequence). Similarly, "Match" and "Unmatch" in the Edit menu of the
- Aligner stack allow a rapid toggling between match and no match characters. A
- number of other conversion options are provided from "Nucleotide strings" or
- "Peptide strings..." under the Convert menu for importing single or multiple
- sequences back into EuGene, CLUSTAL or various other molecular biology software
- packages.
-
- Besides converting either GenBank or EMBL format sequences to strings, you can
- also convert them to "smart" gene maps, example of which are found on the
- gene mapping cards of DNA Translator. This depends on the standard documentation
- accompanying many GenBank/EMBL sequences which lists various features by
- coordinates. This optional conversion is performed during the normal conversion
- (e.g., SD #7 -> #8), but the matrix produced then needs to be imported as a
- "New Card" from any gene mapping card.
-